ROLE OF SELENIUM AND VITAMIN E IN LACTOSE BASED EXTENDER ON SEMEN CRYOPRESERVATION OF BUFFALO BULL (BUBALUS BUBALIS)


 This study was designed to evaluate the supplementation of various concentrations selenium and vitamin E combinations in lactose egg-yolk-glycerol based extender on cryosurvival buffalo bull semen. The semen four healthy donor bulls ejaculates (n= 80) were processed pooled. collected extended with experimental extenders then frozen. frozen straws thawed analyzed for motility, viability, acrosomal integrity functional sperm. indicated that combined use @ 2μg/ml + 0.75 mg/ml supplemented had significantly (P<0.05) increased sperm membrane integrity. Selenium (2μg/ml) freezing also improved cryosurvival. provision (3μg/ml) alone combination Vitamin (1 mg/ml) did not provide any beneficial impact It concluded addition fructose egg yolk glycerol sperms.